Journal: STAR Protocols

Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

doi: 10.1016/j.xpro.2021.100868

Figure Lengend Snippet:

Article Snippet: 96 Floating E-Clip style Pin Multi-Blot Replicator , V&P Scientific , VP 408FS2AS.

Techniques: Recombinant, Clone Assay, Software, Microscopy

Journal: Frontiers in Microbiology

Article Title: Genome-Wide Identification of Host Genes Required for Toxicity of Bacterial Cytolethal Distending Toxin in a Yeast Model

doi: 10.3389/fmicb.2019.00890

Figure Lengend Snippet: Screening of the yeast deletion library for mutations that confer CdtB resistance. Representative images of yeast deletion mutants carrying pYES-CdtB spotted on glucose (repressing media) and on galactose (inducing media). (A) Primary screening of the yeast deletion library was carried out using a 96-pin replicator. An example of the screen of a library plate is shown (library plate no. 4). The mutants with dashed circles were selected for secondary screening. (B) Secondary screening was performed by spotting dilutions of mutants that showed resistance in the primary screen. The 513 mutants from the primary screening were compiled in 96-well plates and dilutions of cultures were spotted on solid media. An example of a compilation plate at 1:200 dilution is shown (plate R2). The last well (circled) was the wild-type as a control. (C) Confirmatory test was performed using spot tests of 10-fold serial dilutions of mutants in comparison to the wild-type control. Three examples of CdtB resistant mutants that showed better growth than wild-type at various levels are shown. Plates were photographed after approximately 40 h of incubation at 30°C.

Article Snippet: The cultures were serially diluted and inoculated with a 96-pin floating pin replicator (V&P Scientific, Inc., United States) on SC-Ura media containing 2% galactose (inducing media) and on that containing 2% glucose (repressing media) as a control.

Techniques: Incubation

Journal: Methods (San Diego, Calif.)

Article Title: Determining the fidelity of tRNA aminoacylation via microarrays

doi: 10.1016/j.ymeth.2016.09.004

Figure Lengend Snippet: The station shown is underneath a small plexiglass hood to prevent dust accumulation and also to control humidity during the printing process. During array printing, the Floating Pin Replicator dips its pins into the 384-well printer plate containing the tRNA probes. The Glass Slide Indexing System then guides the pins of the Floating Pin Replicator to apply the probes to precise locations on the array. The Glass Slide Replicator Wash & Blot station is used to wash the pins of the Floating Pin Replicator when new section of the 384-well printer plate is being utilized in order to prevent probe cross-contamination. The Replicator Pin Drier accelerates the pin drying after they are washed.

Article Snippet: First, the Floating Pin Replicator (V&P Scientific, VP478A) has 24 individual pins that dip into a 384-well plate containing the tRNA probes and transfers a small volume of diluted oligonucleotide solution (~10 nl) to the glass array.

Techniques: Control